The French forward suffered the injury during a victorious match against Fulham at Old Trafford, with a score of 2-1, last Sunday.
Following medical examinations, the club confirmed on Tuesday that the 27-year-old forward had sustained a muscle tear.
If Manchester United triumphs on Saturday, it would secure a remarkable domestic double for the club, as they had previously won the League Cup in February.
Victory in the final would prevent their city rivals from matching the historic Treble achieved by Sir Alex Ferguson’s Manchester United team in 1999.
It is worth noting that the reigning Premier League champions, Manchester City, will also be competing in the Champions League final next month.
Throughout the season, which has been plagued by injuries, Martial contributed significantly with six goals and two assists in 21 Premier League matches.
Additionally, Manchester United had concerns about the fitness of winger Antony, who was stretchered off in tears during their 4-1 triumph over Chelsea.
However, manager Erik ten Hag expressed optimism on Sunday, stating that the 23-year-old Brazilian is likely to recover from his ankle injury in time for the final on Saturday.